It is intended for the collection, transport, and storage of blood and stabilization of intracellular RNA in a closed tube and subsequent isolation and purification of intracellular RNA from whole blood for RT-PCR used in molecular diagnostic testing.
Performance characteristics of the PAXgene Blood RNA System have been established with FOS and IL1B gene transcripts.
At each indicated time point, RNA was purified from duplicate tubes with the PAXgene Blood RNA Kit and analyzed using a quantitative RT-PCR assay specific for IL1B.
Red dashed lines represent assay precision (±3× total precision of the assay with consideration of single data; |3 × σT| = 1.98 CT).
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The user is responsible for establishing appropriate PAXgene Blood RNA System performance characteristics for other target transcripts.